Figure 5.

Effect of different TLR9-agonists on release of microvesicles from peripheral blood mononuclear cells. Peripheral blood mononuclear cells from healthy donors (n = 7) were incubated for 24 h with ODN2006, a weak inducer of IFN-α, or ODN2395, a strong inducer of IFN-α. Microvesicles (MVs) released into the culture supernatants were subsequently isolated by differential centrifugation. Isolated MVs were quantified and characterized with respect to expression of G3BP and dsDNA by flow cytometry. (A) Shown is the consequent increase in the concentration of MVs expressing G3BP (white columns), dsDNA (gray columns), or both (black columns). (B) The concentration of G3BP-expressing MVs in cultures of stimulated cells relative to that in non-stimulated cultures (NS). (C) The corresponding relative concentration of dsDNA-expressing MVs. Columns and error bars represent median values and interquartile range. *P < 0.05. NS, non-stimulated.