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. 2019 Oct 11;10:1135. doi: 10.3389/fphar.2019.01135

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Copyright © 2019 Wen, Zhang, Liu, Wang, Li, Yang, Wang, Cai, Li and Zhao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative photomicrographs and summary data for HCS image analysis of H9c2 cells. (A) Representative photomicrographs for HCS analysis of H9c2 cells; scale bar = 50 μm. (B) Valid cell count for HCS analysis of H9c2 cells (% of control). (C) Live cell count (MEAN_TargetAvgIntenCh2). (D) Dead cells count (MEAN_TargetAvgIntenCh3). ^* P < 0.05, ^** P < 0.01 versus control group, ^# P < 0.05, ^## P < 0.01 versus DOX group. Results are expressed as percentages of control group. Data are shown as mean ± SD (n = 6). HCS, high-content screening; SAL, salsolinol; DOX, doxorubicin; EthD-1, ethidium homodimer-1.