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. 2019 Oct 11;10:1135. doi: 10.3389/fphar.2019.01135

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Copyright © 2019 Wen, Zhang, Liu, Wang, Li, Yang, Wang, Cai, Li and Zhao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative images and corresponding numerical data for some typical cytotoxic effects of SAL on H9c2 cells nuclei and mitochondria. (A) Representative photomicrographs and summary data for TMRE staining of H9c2 cells. Scale bar = 50 μm. (B) Effect of different drugs on valid object cell count of H9c2 cells. (C) Effect of different drugs on nuclear size of H9c2 cells (MEAN_ObjectSizeCh1). (D) Effect of different drugs on average brightness of functional mitochondrial mass (A). (E) Effect of different drugs on projected area occupied by functioning mitochondrial mass (MEAN_TargetAreaCh2). ^** P < 0.01 versus control group, ^# P < 0.05, ^## P < 0.01 versus DOX group. All data are presented as mean ± SD (n = 6). SAL, salsolinol; DOX, doxorubicin.