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. 2019 Oct 11;13:1092. doi: 10.3389/fnins.2019.01092

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Copyright © 2019 Stroh, Kressel, Coras, Dreyer, Fröhlich, Förschler, Lobsien, Blümcke, Zoubaa, Schlegel, Zimmer and Boltze.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytological analysis of magnetically labeled haNSCs (n = 3 with 3 technical replicates each). (a) Unlabeled control cells and (b) intracytoplasmic VSOP uptake by haNSCs following incubation with 0.5 mM VSOP. (c–f) Cells were fixed with 4% phosphate-buffered saline-buffered paraformaldehyde and intracellular iron was visualized using Prussian blue staining on day 2 as shown in (c,e) and on day 28 as provided in (d,f) after labeling. (g) Cell counting revealed that labeling efficacy at any time point could not be enhanced significantly by lipofection. (h) Proliferation analysis of VSOP-labeled haNSC (1.5 mM) revealed no statistically significant difference in the proliferation abilities of unlabeled haNSC and haNSCs labeled with 0.5 mM VSOP, respectively. Scale bars in (a–f) represent 10 μm. ^∗p < 0.01.