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. 2019 Oct 11;10:977. doi: 10.3389/fgene.2019.00977

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Copyright © 2019 Marín-Llera, Garciadiego-Cázares and Chimal-Monroy

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Undifferentiated zones beneath limb ectoderm as reservoirs of stem/progenitor cells. (A) Scanning electron microscopy of a sagittal section of a 23HH chicken forelimb showing the undifferentiated zone. Yellow and orange squares-marked regions represents the image showed in B and C respectively. (B) Magnification of yellow boxed area. The undifferentiated zone, in purple, is under the dorsal ectoderm (yellow) influence. The region where mesenchymal cells are committed to chondrogenic/tenogenic lineage is showed in orange. (C) Magnification of orange boxed area. The undifferentiated zone (purple) underlies the Apical Ectodermal Ridge (AER) and dorsal and ventral ectoderm (yellow). Notice that the marginal vein, indicated with an asterisk, delimits the undifferentiated zone and the committed zone (orange).