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Chondrogenesis starts when the limb mesenchymal cells leave the influence of the AER-FGFs signalling. (A). Histological section of a silver-stained 28-HH chicken digit showing a cartilage condensation. (B). In situ hybridization for Sox9 (a,c) and blood vessel cell-tracker staining (b,d) in chicken hindlimbs at 25HH and 29HH stages. Noticed that cartilage differentiation is accompanied by vascular regression. Avascular zones can be observed at the later stage in the digits.
