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. 2019 Oct 11;10:1268. doi: 10.3389/fpls.2019.01268

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Copyright © 2019 Huang, Li, Liu, Zhu, Zhao, Zhou, Zhang, Wu, Dai, Xia and Gao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantification of 3-DHS and SA production catalyzed by recombinant CsDQD/SDHs in vitro. The reaction products in enzymatic assays were quantified using HPLC (λ _3-DHS = 234 nm, λ _SA = 211 nm). Data are shown as the mean of three replicates ± SD. Different letters represent statistically differ at P < 0.05 according to one-way ANOVA of Duncan’s test. 3-DHS, 3-dehydroshikimate; SA, shikimate; HPLC, high-performance liquid chromatography; ANOVA, analysis of variance.