Table 1.
Michaelis–Menten kinetic parameters of recombinant CsDQD/SDHs for each substrate.
| Enzyme | Substrate | Product | KM (μM) | Vmax (nKat·μg-1) | kcat (S-1) | kcat/KM (S-1·M-1) |
|---|---|---|---|---|---|---|
| CsDQD/SDHa | NADPH | SA | 270.306 | 31.545 | 0.202 | 746.282 |
| 3-DHS | 286.576 | 63.291 | 0.405 | 1412.281 | ||
| NADP+ | 3-DHS | 138.031 | 18.248 | 0.117 | 845.397 | |
| SA | 272.782 | 23.981 | 0.153 | 562.168 | ||
| CsDQD/SDHb | NADPH | SA | nd* | |||
| 3-DHS | nd | |||||
| NADP+ | 3-DHS | nd | ||||
| SA | nd | |||||
| CsDQD/SDHc | NADPH | SA | 267.244 | 7.987 | 0.052 | 193.409 |
| 3-DHS | 330.933 | 25.907 | 0.168 | 506.596 | ||
| NADP+ | 3-DHS | 114.553 | 6.579 | 0.043 | 371.655 | |
| SA | 199.653 | 16.529 | 0.107 | 535.744 | ||
| CsDQD/SDHd | NADPH | SA | 248.798 | 7.758 | 0.049 | 196.708 |
| 3-DHS | 465.971 | 7.353 | 0.046 | 99.546 | ||
| NADP+ | 3-DHS | 46.342 | 0.937 | 0.006 | 127.616 | |
| SA | 610.643 | 13.405 | 0.085 | 138.482 |
nd, no activity detectable or too low to determine kinetic properties.