FIGURE 2.

Lm-crlf3 expression in hemocytes, brain and skeletal muscle. (A) 1% Agarose gel of RT-PCR products (using primers for Lm-crlf3 fragment 1 and 100 ng cDNA template) from hemocytes (H), brain (B), and skeletal muscle (M) extracted from adult and juvenile locusts. (B) qRT-PCR analysis of crlf3 expression in hemolymph, brain, and skeletal muscle of adult locusts. Lm-gapdh was used as a reference gene and relative expression of Lm-crlf3 was normalized to the level of expression in the brain (set to 1). Bars display average relative expression level ± standard deviation. N = 3.