FIGURE 3.

Single gene tree based on 293 amino acid sequences referring to CRLF3. CRLF3 is a well-conserved ancient protein present in eumetazoan taxa ranging from Cnidaria to Mammalia. No CRLF3 was detected in the basal metazoan taxa of Porifera, Placozoa, and Ctenophora. The topology resembles the phylogenetic relationships concluded from conventional molecular markers. For reasons of clarity, major taxa were collapsed and only the most important support values depicted as red (100%) and gray (90–99%) nodes. Important events in the evolution of the nervous system and cytokine signaling are indicated at the respective branches. A detailed tree that displays all species individually is presented as Supplementary Figure S1. The scale bar represents the expected number of amino acid substitutions per site.