Table 2.
Hemolytic activity of recombinant and synthesized Px-Mor peptides.
| Peptides | Percentage hemolysis (μM) | |||||||
|---|---|---|---|---|---|---|---|---|
| 100 | 50 | 25 | 12.5 | 6.25 | 3.125 | 1.56 | 0.78 | |
| Px-Mor-His (recombinant) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Px-Mor113/114 (synthesized) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Px-Mor115 (synthesized) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Px-Mor (synthesized) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Melittin (synthesized) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
The hemolytic activity of the peptides was evaluated by determining the hemoglobin release of 8% suspensions of fresh human erythrocytes at 414 nm.