Fig. 3. mRNA expression pattern in superovulated mice.

Whole cumulus GC RNA was isolated from 8-week-old female mice primed with 5 U of PMSG for 48 h at the designated time points after administration of 5 U of hCG. Expression of Cd24a (a), Ptgs2 (b), Pla2g4a (c), Slco2a1 (d), Ptges (e), and Abcc4 (f) was quantified by real-time PCR and normalized to GAPDH expression (n = 10 mice per group). Data are means ± SEMs. The Wilcoxon signed-rank test was used. *p < 0.05 and ***p < 0.001