Skip to main content
. 2019 Oct 17;10(11):791. doi: 10.1038/s41419-019-1995-1

Fig. 5. Depletion of CD24 attenuates EGFR/ERK1/2 signaling cascades.

Fig. 5

a CD24 depletion was verified by western blotting (up) and real-time PCR (down). MOCK untreated COV434 cells, NC negative control, COV434 cells infected with shRNA lentiviral particles with noneffective scramble shRNA sequences; shRNA_1 and shRNA_2, COV434 cells infected with shRNA lentiviral particles with CD24 shRNA_1 or CD24 shRNA_2 sequences. b The effect of CD24 knockdown on the protein stability of EGFR. COV434 cells infected with control shRNA (NC negative control) or CD24 shRNA (shRNA_2) lentivirus were treated with CHX (20 ug/mL) over the course of 12 h. Loss of CD24 greatly decreased the protein stability of EGFR. c The effect of CD24 depletion on EGF-driven ERK1/2 signaling cascades in COV434 cells. Western blot analysis showed that knockdown of CD24 led to the reduction of EGF− induced EGFR/ERK1/2 signaling cascades. Each experiment was performed at least three times