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. 2019 Oct 17;9:14926. doi: 10.1038/s41598-019-51472-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of IBU:LIM (1:4), IBU and LIM in inducing cell cycle arrest and apoptosis on HT29 cells. Cell cycle analysis distribution on HT29 cells after incubation for 24 hours (A). Caspase-3 detection on HT29 cells treated during 24 hours. Active caspase-3 was detected by incubation with NucView488TM (apoptotic cells in green) and viable cells detected by incubation with MitoView633TM (cells with active mitochondria in red) (B). The scale bar is 50 μm. Results are mean ± SD of four (A) and two (B) independent experiments performed in duplicated. Statistical significant differences are expressed in asterisks (β = P ≤ 0.05, ε = P ≤ 0.01, ψ = P ≤ 0.001, ω = P ≤ 0.0001) by one-way ANOVA for multiple comparisons by Dunnett’s method.