Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 17;9:14901. doi: 10.1038/s41598-019-50166-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Cyclic tensile strain (CTS) loading induces CD44 cleavage and de-differentiation of bovine articular chondrocytes (BACs). (A) Western blot analysis showing enhanced CD44 cleavage and decreased SOX9 expression induced by CTS loading (0.5 Hz and 20% elongation) for 12 hours. Enhanced CD44 cleavage was noted as 18–20 kD doublet C-terminal fragment (CD44-EXT) bands and the 15 kD intracellular domain (CD44-ICD) band by the polyclonal anti-CD44 antibodies. (B) Changes in articular chondrocyte-related genes by CTS loading. CTS loading significantly decreased the mRNA expression of chondrocyte differentiation markers (SOX9, aggrecan, and collagen type 2 [COL2]). In contrast, mRNA expression of a chondrocyte de-differentiation marker (collagen type 1 [COL1]) was significantly increased by CTS loading. (C) CTS loading decreased the mRNA expression of SOX9, aggrecan, and COL2 mRNA, and increased the expression of COL1 mRNA, at 12 hours. Values are mean ± standard deviation from six independent experiments. *p < 0.05.