Fig. 8.

Hyper-long telomere mice show less DNA damage and less senescence. a, b Quantification of DNA damage in age-matched (100 weeks old) hyper-long telomere mice and control mice. Quantification of total damage as indicated by percentage of cells with ≥2 53BP1 foci as determined by immunofluorescence (a) and quantification of cells showing telomere-induced DNA damage as shown by percentage of cells with ≥1 TIF (telomere induced foci) as determined by telomere FISH followed by immunofluorescence with 53BP1 antibody (b). c Representative images of TIFs (yellow arrow). d Quantification of the percentage of p21 positive cells in liver of age matched (100 weeks old) hyper-long telomere mice and control mice. e Representative images of p21 (red arrows) positive cells. Error bars represent standard error. t-test was used for statistical analysis. The number of mice is indicated in each case. **p < 0.01. Source data are provided as a Source Data file