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. 2019 Oct 17;9:14907. doi: 10.1038/s41598-019-51445-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) In vitro specificity of [¹¹¹In]In-DOTA-HE₃-ZCAIX:2 binding to renal cell carcinoma SK-RC-52 cell line. In blocking group, receptors were pre-saturated by 100-fold excess of nonlabeled HE₃-ZCAIX:2. (B) Internalization of [¹¹¹In]In-DOTA-HE₃-ZCAIX:2 by renal cell carcinoma SK-RC-52 cells during continuous incubation. Cells were incubated with conjugate (10 nM) at 37 °C. Data are normalized to the highest cell-bound activity and presented as mean values from 3 cell dishes and SD. Error bars might be not seen because they are smaller than symbols.