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. 2019 Oct 17;9:14922. doi: 10.1038/s41598-019-51443-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of RNA-Seq results by RT-qPCR. Correlation between RNA-Seq and RT-qPCR data. The expression of eight miRNAs was validated using RT-qPCR. Letters A to H represent the miRNA names novel-mir001, novel-mir007, novel-mir013, novel-mir017, bra-miR160a-3p, bra-miR159a, bra-miR158-5p and bra-miR164a, respectively. Numbers 1, 6 and 12 represent the corresponding time-points of 1 h, 6 h and 12 h. The RT-qPCR log₂-fold change values (X-axis) were plotted against RNA-seq data (Y-axis).