Fig. 6.
High cytosolic calcium levels promotes nuclear translocation of TFEB in the absence of spatacsin. a Western blot of TFEB in cytosolic and nuclear fractions of Spg11+/+ and Spg11−/− fibroblasts cultured for 2 h in normal medium or medium containing either 0.4 mM CaCl2 or 0.5 µM EGTA-AM. Graphs show the quantification of the amount of TFEB normalized to the levels of α-tubulin (Cytosol) and Histone H3 (Nuclei). One-way ANOVA: *p < 0.05, **p < 0.01. b Western blots of TFEB in cytosolic and nuclear fractions of Spg11+/+ and Spg11−/− fibroblasts transfected with control siRNA or a specific siRNA that downregulates calcineurin (Calci). Downregulation of calcineurin is evidenced by western blot in the cytosolic fraction. c Quantification of the amount of nuclear TFEB normalized to the levels of Histone H3 upon downregulation of calcineurin (SiCalci). One-way ANOVA: *p < 0.05