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. 2019 Oct 17;9:14915. doi: 10.1038/s41598-019-51560-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of ROS treatment on TEER values and intercellular junctions of HUVECs monolayer on the Col-ANM. (A) Change in TEER values over time after ROS treatment. (B) Immunofluorescence images of HUVECs after ROS treatment: before ROS treatment (B-i), 15 min after ROS treatment (B-ii), and 60 min after ROS treatment (B-iii). Adherens junctions and tight junctions were stained with VE-cadherin (red) and ZO-1 (green), respectively, and nuclei were stained with DAPI (blue). Scale bars are 20 μm.