Fig. 7.

Liver-specific Tmem127 deletion increases hepatic and peripheral insulin sensitivity. a Relative liver, muscle, and iWAT mRNA expression of the indicated glucose transporter and glycolysis genes from Flx (n = 5) and LKO (n = 11) adult mice (9–12 mo old); b western blot of liver and inguinal white adipose tissue lysates from chow-fed LKO and Flx adult male mice, probed with phosphorylated Ser473 and Thr308 Akt and total Akt; c relative liver mRNA expression of the indicated fatty-acid synthesis genes from LKO and AKO adult male mice, represented as ratio over Flx (n = 12 Flx, 11 LKO and 7 AKO, 9–12 mo); d relative liver mRNA expression of the indicated lipogenic transcription factor genes from LKO and AKO adult male mice (n = 11 Flx, 12 LKO and 7 AKO, 9–12 mo). e Real-time (RT) PCR of liver G6pase gene expression in control or AKO 9–12 mo old male mice starved of food for 6 h and re-fed for 3 h (fed) or starved for 24 h (fasted), mice or feed condition (WT n = 4, KO n = 7); f RT-PCR of liver of the indicated fatty acid synthesis genes from chow-fed, adult male Flx (n = 11), and AKO (n = 5) mice; g RT-PCR of liver of the indicated transcription factor genes from adult male Flx (n = 11) and AKO (n = 5) mice; h RT-PCR of inguinal fat (iWAT) Glut4 gene expression from chow-fed, adult male Flx (n = 11) and AKO (n = 5) mice; i western blot of inguinal white adipose tissue lysates from chow-fed LKO and Flx adult male mice (n = 3 per genotype), probed with total ACC and a loading control; j respiratory quotient (RQ) of Flx (n = 5) or AKO (n = 7) chow-fed, adult male mice, data collected for 48 h and sorted by day or night cycle. Data were analyzed by Student’s t test. Values are expressed as mean ± s.e.m. *P < 0.05; **P < 0.01. See also Supplementary Fig. 7A–C. Source data are provided as a Source Data file