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. 2019 Oct 17;9:14916. doi: 10.1038/s41598-019-51287-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NKF-NK cells exhibit potent cytotoxic activity against both hematologic and solid cancer cells. The cytotoxic activity of 2 week expanded NKF-NK or IL-2-NK cells was assessed against hematologic malignancy cell lines (A) or solid tumor cell lines (B) after 4 hr of co-culture at a 1:1 NK:Target cell ratio, n = 4. (C) NKF-NK cells demonstrate dose-dependent cytotoxicity against cancer cells. The cytotoxic activity of NKF-NK cells was measured using the indicated NK:Target ratios, n = 4. Data represents mean +/− SEM. *p < 0.05, **p < 0.01.