Fig. 5.

MORF_DPF has DNA-binding activity. a Electrostatic surface potential of the MORF_DPF in complex with H3K14cr is shown. Blue and red colors indicate positive and negative charges, respectively. b EMSA of 147-bp 601 DNA incubated with increasing amounts of WT MORF_DPF. DNA to protein molar ratio is shown below the gel image. c–e EMSA of H3K14cr-NCP incubated with increasing amounts of WT or mutant GST-MORF_DPF. H3K14cr-NCP to protein molar ratio is shown below each gel image. f A schematic showing the dual interaction of MORF_DPF with histone tail and DNA