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. 2019 Oct 17;10(11):790. doi: 10.1038/s41419-019-2019-x

Fig. 3. The total number of oocytes in the ovaries with XIAP deficiency or overexpression at 19.5 and 23.5 dpc.

Fig. 3

A Confocal images of wholemount ovaries, IF-stained with TRA98 (green), anti-TAp63α (red), and anti-γH2AFX (cyan) antibodies with DAPI counterstaining (blue). The results from Xiap+/+ ovaries and those from the non-transgenic ovaries were combined as WT ovaries for presentation purpose. The merged image in 3D stacks is followed by a central Z-section, γH2AFX-staining alone, and the area of the central Z-section (indicated with a white box) at a higher-magnification (High-Mag). Scale bar, 100 μm. B The total number of oocytes per ovary. Green and red indicates the oocytes stained for TRA98 and TAp63α alone, respectively, while yellow indicates the oocytes stained for both TRA98 and TAp63α. C The percentage of γH2AFX-positive oocytes. Data are shown as mean ± SEM. The number of females examined is given above each column. *P < 0.05, **P < 0.01, statistical significance by t-test