TABLE 2.
Proteins modulated during osmoadaptation belonging to the COG category “amino acid metabolism and transport,” “carbohydrate transport and metabolism,” “lipid transport and metabolism” and “cell wall-membrane-envelope biogenesis.”
| Accession | Description | YEL+NaCl/YEL ratio |
| Amino acid transport and metabolism | ||
| emb|CDP48583.1| | Prephenate dehydrogenase | 2.90 |
| emb|CDP47878.1| | ATP-binding protein opuCA of Glycine betaine/carnitine/choline ABC transporter | 2.22 |
| emb|CDP48936.1| | Aminopeptidase | 2.02 |
| emb|CDP48564.1| | polar amino acid ABC transporter, ATP binding component | 1.86 |
| emb|CDP47695.1| | Nucleoside-diphosphate kinase | 1.82 |
| emb|CDP48687.1| | Glycine cleavage H-protein (lipoate-binding) | 1.79 |
| emb|CDP48582.1| | Cytidylate kinase (CK) (Cytidine monophosphate kinase) (CMP kinase) | 1.78 |
| emb|CDP49472.1| | ABC-type choline/glycine betaine transport, ATP-binding protein | 1.67 |
| emb|CDP49173.1| | Dihydroxy-acid dehydratase | 1.65 |
| emb|CDP49146.1| | L-asparaginase I | 1.65 |
| emb|CDP48643.1| | Tryptophan synthase alpha chain (TrpA) | 1.55 |
| emb|CDP47760.1| | Kinase ArgK | 1.52 |
| emb|CDP49589.1| | Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase related deacylase | 1.51 |
| emb|CDP47866.1| | Serine acetyltransferase | 1.51 |
| emb|CDP48503.1| | Amidohydrolase (Peptidase M20D) (Putative metal-dependent amidase/aminoacylase/carboxypeptidase) | 0.67 |
| emb|CDP48372.1| | Binding protein of oligopeptide ABC transporter (OPN: undef: Oligopeptides) | 0.65 |
| emb|CDP49606.1| | Shikimate 5-dehydrogenase | 0.64 |
| emb|CDP49535.1| | cysteine synthase 2 | 0.54 |
| emb|CDP49386.1| | Phospho-2-dehydro-3-deoxyheptonate aldolase | 0.50 |
| emb|CDP47931.1| | Solute binding protein of the ABC transport system | 0.49 |
| Carbohydrate transport and metabolism | ||
| emb|CDP49182.1| | iolB (Myo-inositol catabolism IolB protein) | 2.04 |
| emb|CDP49834.1| | Ribose-5-phosphate isomerase 3 | 1.90 |
| emb|CDP48739.1| | Phosphoketolase pyrophosphate | 1.87 |
| emb|CDP48882.1| | Phosphoglycerate mutase/fructose-2,6-bisphosphatase | 1.84 |
| emb|CDP49742.1| | Endonuclease | 1.68 |
| emb|CDP48781.1| | Alpha-glucan phosphorylase | 1.63 |
| emb|CDP48893.1| | Oxidoreductase | 1.62 |
| emb|CDP47720.1| | 6-phosphogluconate dehydrogenase, decarboxylating | 1.56 |
| emb|CDP49513.1| | Alpha-1,4-glucosidase | 1.55 |
| emb|CDP49568.1| | Polyphosphate glucokinase | 0.66 |
| emb|CDP48902.1| | Fructose-bisphosphate aldolase class I | 0.63 |
| emb|CDP49639.1| | Gluconate kinase (Gluconokinase) | 0.59 |
| emb|CDP47629.1| | Glycogen debranching enzyme GlgX | 0.49 |
| emb|CDP47859.1| | Binding protein of ribose ABC transporter | 0.46 |
| emb|CDP48824.1| | Dihydroxyacetone kinase | 0.43 |
| emb|CDP49430.1| | Glucose-1-phosphate adenylyltransferase (ADP-glucose synthase) (ADP-glucose pyrophosphorylase) (ADPGlc PPase) | 0.39 |
| emb|CDP49099.1| | PTS system, mannose/fructose/sorbose family, IIA component subfamily | 0.26 |
| Lipid transport and metabolism | ||
| emb|CDP49767.1| | Inositol-1-phosphate synthase | 1.87 |
| emb|CDP47827.1| | Enoyl-CoA hydratase/carnithine racemase CaiD | 1.84 |
| emb|CDP49388.1| | Acyltransferase PlsC | 1.52 |
| emb|CDP48433.1| | Acyl carrier protein (ACP) | 0.59 |
| emb|CDP48431.1| | Carboxylic ester hydrolase | 0.57 |
| Cell wall/membrane/envelope biogenesis | ||
| emb|CDP47662.1| | GTP-binding protein LepA | 2.32 |
| emb|CDP49368.1| | UDP-N-acetylmuramoyl-tripeptide–D-alanyl-D-alanine ligase (UDP-MurNAc-pentapeptide synthetase) (D-alanyl-D-alanine-adding enzyme) | 1.98 |
| emb|CDP49372.1| | S-adenosyl-L-methionine-dependent methyltransferase mraW | 1.97 |
| emb|CDP49370.1| | Cell division protein FtsI (penicillin-binding protein 2) (Peptidoglycan glycosyltransferase) | 1.88 |
| emb|CDP47743.1| | UDP-N-acetylmuramyl tripeptide synthase (Mur ligase) | 1.76 |
| emb|CDP49363.1| | UDP-N-acetylmuramate–L-alanine ligase (UDP-N-acetylmuramoyl-L-alanine synthetase) | 1.55 |
| Post-translational modification, protein turnover, and chaperones | ||
| emb|CDP48339.1| | Heat shock protein 20 3 (20 kDa chaperone 3) | 3.25 |
| emb|CDP49617.1| | Thiol peroxidase | 2.50 |
| emb|CDP49795.1| | Thioredoxin | 2.49 |
| emb|CDP49065.1| | Surface layer protein A (S-layer protein A) | 2.24 |
| emb|CDP49048.1| | Thioredoxine | 2.15 |
| emb|CDP49400.1| | HesB protein | 1.92 |
| emb|CDP48411.1| | SmpB SsrA-binding protein | 1.92 |
| emb|CDP48424.1| | Heat shock protein 20 2 (20 kDa chaperone 2) | 1.68 |
| emb|CDP48051.1| | Protein GrpE 2 (HSP-70 cofactor 2) (Co-chaperone protein GrpE2) | 1.62 |
| emb|CDP49021.1| | Protein GrpE 1 (HSP-70 cofactor 1) (Co-chaperone protein GrpE1) | 1.61 |
| emb|CDP48340.1| | Heat shock protein 20 1 (20 kDa chaperone 1) | 1.57 |
| emb|CDP47983.1| | Peroxiredoxin/Alkyl hydroperoxide reductase subunit C/Thioredoxin peroxidase/Alkyl hydroperoxide reductase protein C22/General stress protein 22 | 1.53 |
| emb|CDP49312.1| | FeS assembly protein SufB | 0.61 |
| emb|CDP49702.1| | Stomatin/prohibitin | 0.60 |
| emb|CDP47885.1| | Putative O-sialoglycoprotein endopeptidase | 0.59 |
| emb|CDP48273.1| | Surface layer protein B (S-layer protein B) | 0.41 |
| emb|CDP48858.1| | Surface protein with SLH domain | 0.15 |
Proteins were determined by using a database composed of proteome of P. freudenreichii CIRM-BIA 129 (downloaded from NCBI.nlm.nih.gov 2018). Ratio were calculated using the XIC methods and indicate induction (ratio > 1.5) or repression (ratio > 0.66) by NaCl.