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. 2019 Oct 11;7:262. doi: 10.3389/fbioe.2019.00262

Figure 3.

Figure 3

Ergothioneine production by yeast strains expressing human ERG transporter SLC22A4X or a putative ERG transporter from M. smegmatis on three variations of synthetic complete medium: (A) AA: 20 g/l glucose and 1 g/l of each L-His, L-Met, and L-Cys, (B) Glu: 40 g/l glucose, (C) FiT: 60 g/l EnPump substrate for slow glucose release to simulate fed-batch conditions, (D) Microscope images of yeast cells with ergothioneine transporters linked to GFP. To the left are Brightfield images and to the right GFP images. Top panels; ST8461, ergothioneine producing strain without transporter. Middle-top panels, ST8921, ergothioneine producing strain with a putative transporter from Mycobacterium smegmatis linked to GFP at the N-terminus. Middle panels, ST8922, ergothioneine producing strain with a putative transporter from Mycobacterium smegmatis linked to GFP at the C-terminus. Middle-bottom panels, ST8923, ergothioneine producing strain with a transporter from Homo sapiens linked to GFP at the C-terminus. Bottom panels, ST8924, ergothioneine producing strain with a transporter from Homo sapiens linked to GFP at the N-terminus.