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. 2019 Oct 4;10:1262. doi: 10.3389/fphys.2019.01262

FIGURE 1.

FIGURE 1

RT-PCR analysis of Tmem16a and Cftr mRNA expression in isolated epithelial cells from proximal and distal colon of WT (Tmem16afl/fl) and KO (Tmem16afl/flVil1Cre) mice. (A) Expression of Tmem16a (16a) and Cftr mRNA was not different in isolated epithelial cells of proximal (WT PC) and distal colon (WT DC) of WT (Tmem16afl/fl) mice. Knockout of TMEM16a did not affect mRNA expression of Cftr mRNA in isolated epithelial cells from proximal (KO PC) and distal colon (KO DC) of Tmem16afl/flVil1Cre mice. (B) Quantification of Tmem16a (16a) and Cftr mRNA expression to Glyceraldehyde 3-phosphate dehydrogenase (Gapdh) mRNA expression in isolated epithelial cells of proximal and distal colon. #significant different (t-test) (number of measurements).