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. 2019 Oct 4;10:1262. doi: 10.3389/fphys.2019.01262

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Copyright © 2019 Kunzelmann, Centeio, Wanitchakool, Cabrita, Benedetto, Saha, Hoque and Schreiber.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tmem16a protein is expressed in murine intestinal epithelium. Western blotting of Tmem16a in isolated intestinal crypts. (A,B) No bands for Tmem16a could be detected in two different laboratories using the Tmem16a antibody produced by Abcam (Cat#ab64085), while Tmem16a was detected by the antibody from Alamon labs (Cat#ACL-011). (C) Detection of Tmem16a by the anti-TMEME16A antibody #P49 from Davids Biotechnology (Regensburg, Germany) in distal colon, but not in small intestine, similar to Benedetto et al. (2019b). (D) Using the Tmem16a antibody kindly provided by Harfe and Rock (Romanenko et al., 2010), expression of Tmem16a was found in intestine of three different WT animals, but not in intestine of two different KO animals. Note that this antibody produced a non-specific band at around 100 kDa but was otherwise very clean. Blotting with anti-ß-actin antibody demonstrated equal loading of the lanes. Little Tmem16a was expressed in murine kidney.