TABLE 1.

Primers used for semiquantitative RT-PCR.

Gene accession number	Primer	Size (bp)
Tmem16a (Ano1)	s: 5′-GTGACAAGACCTGCAGCTAC	406
NM_178642	as: 5′-GCTGCAGCTGTGGAGATTC
Tmem16b (Ano2)	s: 5′-CCAGAGGAAAGTCGACTATG	544
NM_153589	as: 5′-GGTAGCATTGTCAAAGAAGG
Tmem16c (Ano3)	s: 5′-TGATAAAAGAAACACATTTGAAAAGAA	611
NM_001081556	as: 5′-GAGGCTGATGCTTGTACCAC
Tmem16d (Ano4)	s: 5′-TGGCTTCATTTTTGCTGTTCT	555
NM_178773	as: 5′-GAAGAGCATGCCTGTGTACC
Tmem16e (Ano5)	s: 5′-TCCTGAGGAGGCGTCTTATG	548
NM_177694	as: 5′-CCCAATCTTTTTCTTCCCCTC
Tmem16f (Ano6)	s: 5′-CATACGAATCTAACCTTATCTGC	520
NM_175344	as: 5′-CATTCTCTGTACAGGAGGTAAC
Tmem16g (Ano7)	s: 5′-TTGGAATCCGAAATGAGGAG	584
NM_207031	as: 5′-GTGTGCGGAGGTGAAAGTG
Tmem16h (Ano8)	s: 5′-CTTGGAGGACCAGCCAATC	682
XM_889480	as: 5′-CTTCTTGTAGCCCTCAGCAC
Tmem16j (Ano9)	s: 5′-CAAGATGTTAAAGGACCAGAAG	487
NM_178381	as: 5′-GAAGATATCATTGGCACTACAG
Tmem16k (Ano10)	s: 5′-GGACATGAAGCTTTTGCGCC	566
NM_133979	as: 5′-TGGCAAATGCGAGTATGAAC
Cftr (Abcc7)	s: 5′-GAATCCCCAGCTTATCCACG	544
NM_021050	as: 5′-CTTCACCATCATCTTCCCTAG
Gapdh	s: 5′-GTATTGGGCGCCTGGTCAC	200
NM_001289726	as: 5′-CTCCTGGAAGATGGTGATGG