Figure 2.

Histochemical analysis of collagen fibre deposits in salivary gland (SG) tissues from healthy controls (a) and primary Sjögren’s syndrome (pSS) (b) patient tissues classified according to different grades of inflammation (I, II, III). Paraffin‐embedded minor salivary glands (MSGs) tissues from non‐specific sialadenitis (non‐SS) were used as positive control (Fig. 2e). The slides were subjected to Masson’s trichrome staining to analyse collagen deposits (blue). At ×20 magnification, a remarkable deposition of collagen fibres were seen in the interstitial area around acinar and ductal cells in pSS SGs tissues (b–d) compared to controls (a). The figure shows an increased collagen deposition from low (b) to high (d) grades of inflammation. Scale bar = 20 μm.