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. 2019 Oct 13;158(3):161–170. doi: 10.1111/imm.13115

Figure 2.

Figure 2

Helios expression. Thymocytes and splenocytes from C57BL/6 mice were analyzed by flow cytometry for the expression of Foxp3 and Helios. Top: CD4 CD8 double‐negative (DN) thymocytes were subdivided based on CD44 and CD25 expression (DN1: CD44+ CD25, DN2: CD44+ CD25+, DN3: CD44 CD25+, DN4: CD44 CD25). Bottom: CD4 CD8 single‐positive thymocytes (left) and CD4+ CD8 splenocytes (right).