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Helios expression. Thymocytes and splenocytes from C57BL/6 mice were analyzed by flow cytometry for the expression of Foxp3 and Helios. Top: CD4− CD8− double‐negative (DN) thymocytes were subdivided based on CD44 and CD25 expression (DN1: CD44+ CD25−, DN2: CD44+ CD25+, DN3: CD44− CD25+, DN4: CD44− CD25−). Bottom: CD4+ CD8− single‐positive thymocytes (left) and CD4+ CD8− splenocytes (right).
