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. 2019 Oct 13;158(3):161–170. doi: 10.1111/imm.13115

Figure 3.

Figure 3

Analysis of heterozygous Foxp3 females. (a) CD4+ Foxp3+ thymocytes or splenocytes from 2‐ or 5‐month‐old Ikzf2fl/fl × Foxp3wt/ YFP ‐Cre mice were analyzed by flow cytometry for YFP expression, a reporter for the Helios‐deficient cells. (b) YFP (wild‐type; WT) or YFP + (knockout; KO) CD4+ Foxp3+ Treg cells were analyzed for CD44 and CD62L to calculate the percent of eTreg cells defined as CD44+ CD62L.