Figure 4.
The effects of BMSCs on the endotheliocytes of alveolar walls in the OS+BMSC group rats. (A) The apoptosis of endotheliocytes in alveolar walls was detected by TUNEL assay. Green fluorescence shows apoptotic cells by a TUNEL assay. Red fluorescence shows the endotheliocytes by a CD34 immunofluorescence assay (magnification, ×400). Scale bar, 100 µm. Nuclei are stained with DAPI. (B) Quantitative analysis of the co-expression of green and red fluorescence, indicating the apoptotic endotheliocytes in the alveolar walls. ***P<0.001, vs. control group; ###P<0.001, vs. OS group.