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. 2019 Sep 24;20(5):4567–4575. doi: 10.3892/mmr.2019.10701

Figure 2.

Figure 2.

RT-qPCR verification of differentially expressed genes identified using microarray analysis and the overexpression of NR_002794 in SWAN71 cells. (A) The expression levels of 10 mRNAs (ARPC3, HCK, PIK3CG, PRKCB, CD209, CLEC4M, FCGR1A, PTPRC, CYBB and NCF4) were determined by RT-qPCR in tissues derived from pre-eclamptic pregnancies and normal pregnancies. (B) The expression levels of 10 lncRNAs (n337689, n340778, n341675, n342887, n345093, n346352, n411602, NR_002794, NR_038877 and NR_039741) were determined by RT-qPCR in tissues derived from pre-eclamptic pregnancies and normal pregnancies. *P<0.05; **P<0.01 vs. respective normal group. (C) Schematic representation of the construction of the overexpression plasmid containing the full-length lncRNA NR_002794. (D) Lentiviral infection of SWAN71 cells. Cells that were infected with lenti-NR_002794 and lenti-con exhibited a consistent infection efficiency. Magnification, ×200. (E) SWAN71 cells were infected with lenti-NR_002794 or lenti-con and the infection efficiency was assessed using RT-qPCR. The data are presented as the mean ± SD. **P<0.01 vs. lenti-con. RT-qPCR, reverse transcription-quantitative PCR; ARPC3, actin related protein 2/3 complex subunit 3; HCK, HCK proto-oncogene Src family tyrosine kinase; PIK3CG, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit γ; PRKCB, protein kinase C β; CD209, CD209 molecule; CLEC4M, C-type lectin domain family 4 member M; FCGR1A, Fc fragment of IgG receptor 1a; PTPRC, protein tyrosine phosphatase receptor type C; CYBB, cytochrome b-245 β chain; NCF4, neutrophil cytosolic factor 4; lenti-NR_002794, lentivirus expressing NR_002794; lenti-con, control lentivirus; fluo, fluorescence.