Figure 3.

DLEU1 knockdown promotes the apoptosis of renal cell carcinoma cells. (A) Flow cytometric analyses were conducted to determine the effects of si-DLEU1 on cell apoptosis in (left) KETR3 and (right) 786-O cells. (B) Post-transfection for 48 h, western blot analysis of apoptosis-associated protein expression (Bcl-2, Bax, C-caspase-3 and C-caspase-9) was conducted in (left) KETR3 and (right) 786-O cells following knockdown of DLEU1. Data are presented as the means ± standard deviation (n=3). Results were obtained from three independent experiments. ^*P<0.05 vs. NC. C-caspase-3, cleaved caspase-3; C-caspase-9, cleaved caspase-9; DLEU1, deleted in lymphocytic leukemia 1; NC, negative control; PI, propidium iodide; si, small interfering RNA.