Figure 4.

DLEU1 knockdown inhibits the Akt pathway and disrupts EMT in renal cell carcinoma cells. (A) Following transfection for 48 h, western blot analysis was conducted to determine alterations in the expression of Akt pathway-associated proteins. (B) Alterations in the expression of marker proteins (E-cadherin, N-cadherin and vimentin) in the EMT process were detected by western blotting. Data are presented as the means ± standard deviation (n=3). Results were obtained from three independent experiments. ^*P<0.05 vs. NC. Akt, protein kinase B; DLEU1, deleted in lymphocytic leukemia 1; EMT, epithelial-mesenchymal transition; NC, negative control; p, phosphorylated; P70S6K, P70S6 kinase; si/siRNA, small interfering RNA.