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. 2019 Sep 10;20(5):4215–4225. doi: 10.3892/mmr.2019.10659

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Upregulated MEG3 suppresses the proliferation, migration and invasion of glioma cells. (A) Transfection efficiency of o/e-MEG3 was determined by RT-qPCR. (B and C) The proliferative activities of GSC11 and D54 cells transfected with o/e- MEG3 or o/e-NC were evaluated using CCK-8 assay. (D and E) The migration of transfected GSC11 and D54 cells were evaluated using a Transwell assay (magnification, ×200). (F and G) The invasion of GSC11 and D54 cells transfected with o/e-MEG3 or o/e-NC were determined (magnification, ×200). *P<0.05. MEG3, maternally expressed 3; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; CCK-8, Cell Counting Kit-8; NC, negative control.