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. 2019 Sep 26;20(5):4674–4682. doi: 10.3892/mmr.2019.10707

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Copyright: © Zou et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Upregulation of the SHH signaling pathway in 16HBECs stimulated by HDM/TGF-β1 for 72 h. (A and B) Western blotting analyses demonstrated that the SHH signaling pathway was induced in 16HBECs by HDM/TGF-β1 treatment for 24–72 h. A representation blot and the quantification are shown. GAPDH was used as an internal control. *P<0.05 and **P<0.01 vs. the control (n=3). (C) mRNA levels of the members of the SHH signaling pathway at different time points in 16HBECs following treatment with HDM/TGF-β1. All values were normalized to GAPDH. *P<0.05 and **P<0.01 vs. control (n=3). SHH, Sonic hedgehog; HBECs, human bronchial epithelial cells; HDM, house dust mite; TGF-β1, transforming growth factor β1.