Figure 4.

Overexpression of miR-195 inhibits the expression of metastasis-associated gene and the activation of the PI3K/AKT signaling pathway in AN3-CA cells. We further examined the effects of miR-195 on the EMT process by measuring the expression of several EMT-associated genes. (A-C) Relative mRNA levels of TIMP-2, MMP-2 and MMP-9 were detected by RT-qPCR. (D and E) Relative protein levels were measured by western blot analysis. (F-H) The protein and phosphorylation levels of PI3K and AKT were detected by western blot analysis. Each value represents the mean ± SEM (n=3). GAPDH was detected as an internal control. ^aP<0.05, ^aaP<0.01 vs. Control group; ^bP<0.05, ^bbP<0.01 vs. Mock group. TIMP-2, tissue inhibitor of metalloproteinase 2; MMP, matrix metalloproteinase.