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. 2019 Sep 6;20(5):4023–4032. doi: 10.3892/mmr.2019.10652

Figure 6.

Figure 6.

GPER was a predicted target of miR-195. In order to investigate mechanisms underlying the inhibition of EC progression by miR-195, we identified the potential target gene of miR-195 in EC cells. (A-C) The transcriptional and translational levels of GPER in AN3-CA cells were detected when cells were transfected with miR-195 mimics. (D-F) The changes in GPER mRNA and protein levels were detected in miR-195 overexpressed Hec1A cells. (G) The fragment of GPER 3′-UTR contains a binding site of miR-195. (H) Dual-luciferase reporter assay was used to reveal whether GPER was a potential target of miR-195. Each value represents the mean ± SEM (n=3). GAPDH was detected as an internal control. aaP<0.01 vs. Control or miR-195 + GPER-3′-UTR group; bbP<0.01 vs. Mock or miR-195 + GPER-3′-UTR mut group. GPER, G protein-coupled estrogen receptor 1.