Figure 1.
Curcumin has no toxic effect on EAEC and EPEC bacterial strains. Bacterial strains EAEC 042 (A) and EPEC E2348/69 (B) were grown in LB broth to an initial optical density of 0.05 (OD600nm), when the bacteria reached an exponential growth phase, different concentrations of curcumin were added (8, 16, 32, 64 μg/mL) for 2 and 4 h at 37°C at 150 rpm. Bacterial strains without curcumin (black bars) or with vehicle DMSO (white bars) served as internal control. The values in all the graphs represent means ± SEM for at least three independent experiments. Statistical significance was determined by using a two-way ANOVA test followed by a Tukey post-hoc test, no significance (ns) (p > 0.05).