Figure 2.

Curcumin inhibits the secretion of SPATEs Pet and EspC (from EAEC and EPEC, respectively) in a concentration- and time-dependent manner. Bacterial cultures of EAEC 042 and EPEC E2348/69 were grown using different curcumin concentrations of (8, 16, 32, 64 μg/mL) for 2 and 4 h at 37°C at 150 rpm. Bacteria were pelleted and the culture supernatants were precipitated for proteins collection (see section Materials and Methods). Protein bands were analyzed by Western-blot using a specific antibody for Pet (A) and EspC (B) passenger domains, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody. As controls, bacterial strains without curcumin or with vehicle (DMSO) were used.