Table 2.
The LOD of OCAv1 assay in regards to the lowest amount of tumor cellularity required for the detection of high level CNAs (>8 copies).
| Gene | Expected copy number | Observed copy number in fresh tumor cell line | Observed copy number in FFPE tumor cell line pooled at various tumor cell percentages | |||||
|---|---|---|---|---|---|---|---|---|
| 100% | 100% | 80% | 50% | 40% | 30% | 20% | ||
| CCND1 | 12 | 24.61 | 13.8 ± 0.44 | 12.58 ± 0.11 | 10.1 ± 2.50 | 8.08 ± 0.02 | 7.82 ± 0.14 | NA |
| MYC | 8 | 7.16 | 7.27 ± 0.25 | 6.33 ± 0.13 | 4.23 ± 0.37 | 4.53 ± 0.11 | 4.96 ± 0.08 | NA |
| AKT1 | 11 | 11.34 | 6.42 ± 0.19 | 5.49 ± 0.04 | 2.57 ± 0.06 | 2.05 ± 0.09 | 1.43 ± 0.06 | NA |
| MDM2 | 9 | 11.27 | 7.9 ± 0.01 | 7.18 ± 0.03 | 6.6 ± 1.04 | 4.89 ± 0.11 | 4.34 ± 0.13 | NA |
| ERBB2 | 9 | 18.77 | 17.11 | 16.36 | 13.48 | 12.98 | 13.63 | 13.1 |