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. 2019 Sep 26;13:1014. doi: 10.3389/fnins.2019.01014

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Copyright © 2019 Carmona, Roudeau, Perrin, Carcenac, Vantelon, Savasta and Ortega.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Procedure for the preparation of adjacent brain cryo-sections. Needle holes orientation marks are made around the region of interest on the tissue block before sectioning. (A) A first brain cryo-section is deposited and maintained between two polycarbonate films stretched over a PEEK sample holder. This first cryo-section will be used for metal analysis. (B) Explanation of how to keep the tissue orientation for correlative imaging. The second cryo-section is used for immunohistochemistry. This second cryo-section is deposited on a glass microscopy slide with the face adjacent to the first one on the top. (C) Illustration of how to identify regions of interest on both sections thanks to the needle holes orientation marks.