Table 1.

The primer sequences, PCR conditions, and restriction enzymes.

Polymorphism	Primer sequences	PCR protocol
GSTA1*C-69T		Denature: 94˚C for 3 min
	F, 5′-GCATCAGCTTGCCCTTCA -3′, R, 5′-AAACGCTGTCACCGTCCTG -3′	Followed by 94˚C for 30 s Annealing: 56˚C for 30 s Extension: 72˚C for 30 s #cycles: 30 Final extension: 72˚C for 10 min Restriction enzyme: Eam1104I incubation at 37˚C overnight
GSTP1*Ile105Val	F, 5′-ACCCCAGGGCTCTATGGGAA-3′, R, 5′-TGAGGGCACAAGAAGCCCCT-3′	Denature: 95˚C for 10 min Followed by 94˚C for 30 s Annealing: 59˚C for 30 s Extension: 72˚C for 30 s #cycles: 29 Final extension: 72˚C for 10 min Restriction enzyme: Alw26I incubation at 37˚C overnight
GSTM1	F, 5′-GAACTCCCTGAAAAGCTAAAGC-3′, R, 5′-GTTGGGCTCAAATATACGGTGG-3′	Multiplex PCR: Denature: 94˚C for 3 min
GSTT1	F, 5′-TTCCTTACTGGTCCTCACATCTC-3′, R, 5′-TCACGGGATCATGGCCAGCA-3′	Followed by 94˚C for 30 s Annealing: 59˚C for 30 s Extension: 72˚C for 45 s #cycles: 30 Final extension: 72˚C for 4 min
CYP1A1	F, 5’-GAACTGCCACTT CAGCTGTCT-3’ R, 5’-CAGCTGCATTTG GAAGTGCTC-3’