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. 2019 Oct 11;12:250. doi: 10.3389/fnmol.2019.00250

FIGURE 3.

FIGURE 3

Modestly suppressed repetitive self-grooming but normal social interaction and sensorimotor function in Lrrc4c–/– mice. (A–D) Normal social approach behavior in Lrrc4c–/– mice (2–5 months; male) in the three-chamber test, as shown by time spent sniffing or time spent in the chamber with social (S1) targets and empty (E) chambers. Note that WT mice exhibit only a modest level of social novelty recognition, showing strongly significant recognition of a novel stranger (S2) when assessed by the time spent in chamber, but not time spent sniffing. n = 9 (WT), n = 11 (KO), ∗∗p < 0.01, ∗∗∗p < 0.001, ns, not significant, two-way repeated-measures ANOVA with Holm-Sidak multiple comparison test [(A) interaction, F(1,36) = 0.128, p = 0.7226; genotype, F(1,36) = 1.087, p = 0.3042; subject, F(1,36) = 141.2, p < 0.001; (B) interaction, F(1,36) = 2.691, p = 0.1096; genotype, F(1,36) = 0.2517, p = 0.6189; subject, F(1,36) = 10.48, p = 0.0026; (C) interaction, F(1,36) = 0.07257, p = 0.7892; genotype, F(1,36) = 0.0068, p = 0.9349; subject, F(1,36) = 289.2, p < 0.001; (D) interaction, F(1,36) = 2.196, p = 0.1471; genotype, F(1,36) = 1.116, p = 0.2977; subject, F(1,36) = 36.93, p < 0.001]. (E) Normal self-grooming in Lrrc4c–/– mice (2–5 months; male) in novel bedded home cages, as shown by total self-grooming duration (10 min). n = 23 (WT), n = 20 (KO), ns, not significant, Student’s t-test [(E) t(41) = 1.559; p = 0.1266]. (F,G) Suppressed self-grooming in Lrrc4c–/– mice (2–5 months; male) in Laboras cages, as shown by time spent in self-grooming (2 days). n = 23 (WT), n = 20 (KO), ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ns, not significant, two-way repeated-measures ANOVA with Holm-Sidak multiple comparison test, Student’s t-test [(F) interaction, F(47,1410) = 2.429, p < 0.0001; time, F(47,1410) = 6.467, p < 0.0001; genotype, F(1,30) = 26.73, p < 0.0001; 2 h, p = 0.0499; 4 h, p = 0.0032; 5 h, p = 0.0143; 27 h, p = 0.0005; 29 h, p = 0.0005; 35 h, p = 0.0499; (G) interaction, F(1,60) = 19.14, p < 0.001; genotype, F(1,60) = 38.99, p < 0.001; time, F(1,60) = 43.19, p < 0.001; OFF, p = 0.1913, ON, p < 0.001]. (H) Normal motor coordination and learning in Lrrc4c–/– mice (2–5 months; male) in the rotarod test. n = 7 (WT), n = 9 (KO), ns, not significant, two-way repeated-measures ANOVA with Holm-Sidak multiple comparison test [(H) interaction, F(4,56) = 0.4294, p = 0.7867; genotype, F(1,14) = 0.163, p = 0.6925; time, F(4,56) = 7.028, p = 0.0001]. (I–K) Normal pre-pulse inhibition (I) and startle responses (J,K) in Lrrc4c–/– mice (2–5 months; male). n = 10 (WT), n = 7 (KO), ns, not significant, two-way repeated-measures ANOVA with Holm-Sidak multiple comparison test, Student’s t-test [(J) t(15) = 0.553; p = 0.5884; (K) t(15) = 0.9855; p = 0.3400].