FIGURE 3.

Transcriptional and functional analysis of DnrH ncRNA in Deinococcus radiodurans. (A) DnrH transcription under heat stress conditions in the WT, ΔDnrH (mutant), and cDnrH (complemented) strains. Total RNA was extracted, and the expression of DnrH was measured by qRT-PCR. (Inset) RNA northern blot assay using RNA extracted from the same strains under the same conditions and hybridized with the DnrH-specific probe. Measurements were normalized to the WT values, and fold differences are plotted. (B) Growth curves of WT, ΔDnrH, and cDnrH in TGY broth. The error bars represent the calculated standard deviation of the measurements of three biological replicates. (C) Serial 10-fold dilutions of OD-standardized cultures were spotted on TGY plates after exposure to 48°C.