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. 2019 Oct 11;10:2354. doi: 10.3389/fmicb.2019.02354

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Copyright © 2019 Xue, Chen, Li, Han, Liu, Jiang, Zhou, Zhang, Chen, Lin, Ongena and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interaction between DnrH and Hsp20 mRNA in vitro and in vivo. (A) Schematic of the interaction between DnrH and Hsp20 mRNA based on IntaRNA. Exemplary interaction between Hsp20 and DnrH. The region of the interaction is depicted in red bold letters. (B) Interactions between FAM-labeled 5′ upstream region of DnrH-wt [green, Kd (wt)], DnrH-mut (red) and Hsp20 mRNA. All interactions were measured using Monolith NT.115 and K_d-values were calculated based on at least three independent replicates using the Nanotemper MO. Affinity Analysis Software. (C) Serial 10-fold dilutions of OD-standardized cultures were spotted on TGY plates after exposure to 48°C.