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. 2019 Oct 11;10:1296. doi: 10.3389/fphys.2019.01296

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Copyright © 2019 Miao, Pan, Wang, Yang, Zhu and Gong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ghrelin suppressed while IGF-1 stimulated the differentiation of 3T3-L1 and human primary preadipocytes. Differentiation was measured based on Oil Red O staining revealing triglyceride (A,C,E,G) and G-3-PDH activity (B,D,F,H). Preadipocytes were treated with ghrelin (A–D) and IGF-1 (E–H). The data are presented as the mean ± SD for three independent experiments with eight (A,C,E,G) or three (B,D,F,H) individual wells in each experiment. ^∗p < 0.05 vs. control group.