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. 2019 Oct 11;10:1296. doi: 10.3389/fphys.2019.01296

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Copyright © 2019 Miao, Pan, Wang, Yang, Zhu and Gong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ghrelin inhibited the LPL mRNA levels in 3T3-L1 and human differentiating adipocytes. Levels of LPL mRNA in 3T3-L1 (A) and human adipocytes (B) were measured by RT-PCR after treatment with 10 ng/ml ghrelin. LPL (770 bp) and GAPDH (145 bp) in 3T3-L1 preadipocytes, as well as LPL (227 bp) and β-actin (540 bp) in human adipocytes are shown with molecular markers in the upper panel of (A,B). In the lower panel, data were calculated using the density ratio of LPL to GAPDH (A) and β-actin PCR products (B). The data are presented as the mean ± SD for three independent experiments. ^∗p < 0.05 vs. control group.